Control of Rhizoctonia solani with extracts from Ovidia andina
New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , , , , , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=12070 |
| Sumario: | New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia
solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen.
Powdered dried leaves and stems were extracted by maceration with dichloromethane
(CH2Cl2), methanol and ethanol. An aqueous extract was obtained by decoction, and the
hydrodistillation residual water was the aqueous phase after essences production. A
strain of R. solani was cultivated on potato dextrose agar supplemented with the extracts,
at 1:100 and 1:1000 v/v. The CH2Cl2 extract at 1:100 reduced colony growth (p< 0.0001)
and changed hyphal morphology. Five fractions were obtained from the CH2Cl2 extract in
a glass column, and TLC analysis showed the presence of coumarins and flavonoids. The
CH2Cl2 extract and fractions were diluted in CH2Cl2 or acetone. The efficiency to reduce
pathogen growth of fractions 2f and 5f diluted in CH2Cl2 at 1:100 did not differ from the
whole extract (p<0.0001). For an in vivo test, beetroot seed balls were incubated in soil
infested with R. solani and treated by immersion in the CH2Cl2 extract at 1:100 for different
periods of time. As a result, pathogen colonization was diminished by immersion for 120 s
and seed germination was normal (p< 0.0001). Additionally, infested soil was treated with
the CH2Cl2 extract, and inoculum concentration estimated using beetroot seed balls as
baits diminished (p< 0.0001). These results lead us to conclude that O. andina is a source
of antifungal components for crop protection against R. solani. |
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