Micropropagación de Cissus tiliacea, planta del sur del estado de México
Murashige-Skoog (MS) or Lloyd and McCown (WPM) medium were used for micropropagation of Cissus tiliacea, an herb plant with potential in agriculture and pharmacology; in both medium similar results for shoot regeneration, number of leaves, leaves nodes, and adventitious roots were observed; o...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , , , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=4312 |
| Sumario: | Murashige-Skoog (MS) or Lloyd and
McCown (WPM) medium were used for
micropropagation of Cissus tiliacea, an
herb plant with potential in agriculture and
pharmacology; in both medium similar
results for shoot regeneration, number of
leaves, leaves nodes, and adventitious roots
were observed; only formation of callus
was influenced (p ≤ 0.05) by the medium.
For in vitro multiplication, benzyl adenine
(BA) was added to WPM at 0, 0.5, 1.0, 1.5
or 2.0 mg L-1 and we used three types of
explants that is basal, medium an upper. BA
at 0 and 0.5 mg L-1 resulted in higher size and
well developed plantlets; moreover, those
concentrations allowed 1.2 or 1.6 roots per
vitro plant. Those explants cultured in 1.5 or
2.0 BA resulted in callus development. Type
of explant did not influence the evaluated
explants responses. Rooting was evaluated
in MS adding naphthalene acetic acid (NAA),
indol butiric acid (IBA) or indol acetic acid (IAA)
at 0.5 mg L-1; the higher number of secondary
roots and the diameter of the main root was
induced by NAA, but IBA resulted in longer
main roots. Present results suggested that
micropropagation of C. tiliaceae might be and
alternative for conservation and multiplication
of this wild plant. |
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