Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:

Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding p...

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Publicado en:Revista de la Facultad de Ciencias Agrarias
Autores principales: Aros, Danilo, Infante, Rodrigo, Olate, Eduardo, Valdés, Santiago
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Acceso en línea:https://bdigital.uncu.edu.ar/fichas.php?idobjeto=4523
descriptores_str_mv
Breeding
Brotación
Chile
Irradiación gamma
Mejoramiento genético
Mutación
Mutation
Radio susceptibilidad
Radio susceptibility
Sprouting
todos_str_mv 4482
78
eng
Pontificia Universidad Católica, Chile
UCh_FCA
UCh_FCA
UCh_FCA
autor_str_mv Aros, Danilo
Infante, Rodrigo
Olate, Eduardo
Valdés, Santiago
disciplina_str_mv Ciencias agrarias
description_str_mv Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes.
La irradiación con rayos gamma ha sido ampliamente utilizada como herramienta en mejoramiento genético para obtener nuevos cultivares de especies ornamentales tales como Alstroemeria, en que varios cultivares se han obtenido mediante la irradiación de rizomas. La dosis óptima para una apropiada inducción de mutación debe ser considerada para fines de mejoramiento genético y depende principalmente de la susceptibilidad de la planta. En este estudio, rizomas de Alstroemeria aurea cultivados in vitro fueron irradiados con una fuente de rayos gamma utilizando diferentes dosis para evaluar el efecto directo producido. El daño y número de rizomas en brotación fueron observados y registrados durante 61 días después de la irradiación. Al final de este periodo, los rizomas fueron pesados y la mortalidad fue evaluada. Tanto la mortalidad como el peso aumentaron dependiendo de la dosis. Todos los rizomas irradiados mostraron una brotación anticipada en comparación con el control (0 Gy) y no se observaron diferencias significativas en el número final de brotes después de los 61 días entre los diferentes tratamientos. Se observó blanqueamiento y necrosis en todos los rizomas irradiados, siendo más evidente a altas dosis. El DL50 fue establecido alrededor de 40 Gy y la dosis óptima para inducir mutación se sugiere entre 2,5 y 5 Gy, cuando el crecimiento se redujo en un 50%, y probablemente esta dosis pueda ser utilizada con fines de mejoramiento genético.
titulo_str_mv Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma:
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plantilla_str Artículo de Revista
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container_title Revista de la Facultad de Ciencias Agrarias
journal_title_str Revista de la Facultad de Ciencias Agrarias
journal_id_str r-78
container_issue Revista de la Facultad de Ciencias Agrarias
container_volume Vol. 44, no. 1
journal_issue_str Vol. 44, no. 1
tipo_str textuales
type_str_mv Articulos
title_full Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
title_fullStr Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
title_full_unstemmed Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
description Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes.
dependencia_str_mv Facultad de Ciencias Agrarias
title Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
spellingShingle Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
Breeding
Brotación
Chile
Irradiación gamma
Mejoramiento genético
Mutación
Mutation
Radio susceptibilidad
Radio susceptibility
Sprouting
Aros, Danilo
Infante, Rodrigo
Olate, Eduardo
Valdés, Santiago
topic Breeding
Brotación
Chile
Irradiación gamma
Mejoramiento genético
Mutación
Mutation
Radio susceptibilidad
Radio susceptibility
Sprouting
topic_facet Breeding
Brotación
Chile
Irradiación gamma
Mejoramiento genético
Mutación
Mutation
Radio susceptibilidad
Radio susceptibility
Sprouting
author Aros, Danilo
Infante, Rodrigo
Olate, Eduardo
Valdés, Santiago
author_facet Aros, Danilo
Infante, Rodrigo
Olate, Eduardo
Valdés, Santiago
title_sort Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
title_short Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
url https://bdigital.uncu.edu.ar/fichas.php?idobjeto=4523
estado_str 3
building Biblioteca Digital
filtrotop_str Biblioteca Digital
collection Artículo de Revista
institution Sistema Integrado de Documentación
indexed_str 2023-04-25 00:38
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