Estudio genético y molecular de la respuesta a la vernalización en zanahoria (Daucus carota L.)
Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as early-flowering or annual. The main objective wa...
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2011
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| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=5499 |
| Sumario: | Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial
species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted
to warmer climates require less vernalization and can be classified as early-flowering or
annual. The main objective was determine the genetic of the trait and find genomic regions
involved in carrot vernalization requeriments. We evaluated F1, F2, F3, BC1 and BC2
progenies of an intercross between an annual carrot and a biennial carrot. Observed
segregation ratios confirmed that annual habit is dominant over biennial and is a
monogenic character. When annual and biennial accessions of different geographic sites,
and crosses between them, were evaluated, complete dominance of annual habit and also
variability between annual and between biennial accesions was observed. Working with
BLAST, a molecular method that relies on overall similarity, we could not identify FLC
homologs in carrot genome, a gene that plays a central role in species like Arabidopsis and
Brassicas vernalization response. With the construction of a linkage map a genomic region
linked to vernalization response in carrot was identified. That region was mapped in a
linkage group with 78 molecular markers and flanking markers were at 0.69 and 0.79 cM.
This map could be used to develop molecular markers linked to the trait and also in the
future to start physical mapping and secuencing using a carrot BAC library. |
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