Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosome...
Guardado en:
| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , , , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=6107 |
| descriptores_str_mv |
Agentes antimitóticos Allium cepa Cebolla Colchicina Colchicine Duplicación de genes Ginogénesis Gynogenesis Híbridos Hybrids Onions Plantas dihaploides de cebolla Ploidía Ploidy |
|---|---|
| todos_str_mv |
6005 78 eng ULj UNCuyo FCA UNCuyo FCA UNCuyo FCA UNCuyo FCA |
| autor_str_mv |
Bohanec, Borut Foschi, María Laura Galmarini, Claudio Martínez, Liliana Ponce, María T. |
| disciplina_str_mv |
Ciencias agrarias |
| description_str_mv |
Doubled haploid onion (Allium cepa L.)
plants allow the production of completely
homozygous lines for a later production of hybrids.
The haploid plants are normally produced using in
vitro gynogenesis. The obtained haploid plantlets
must be treated with different agents for doubling
chromosomes. It is necessary to adjust the
concentration and the length of treatment of the
doubling agent. In this case, the effect of 250 and
500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1
amiprophos-methyl during 24 and 48 h was
assessed over the rate of onion haploid plantlets
chromosome doubling. The best duplication
treatment was 250 mg.L-1 colchicine for 48 h,
which yielded 100% of doubled haploid plants. On
the other hand, a positive correlation resulted from
the ploidy level and stomatal size, and a negative
correlation between the level of ploidy and
stomatal density. Significant differences between
the stomatal length, width and density in haploid
and doubled haploid plantlets were observed.
An economical and quick method to test ploidy
level in onion plantlets is proposed through the
measurement of stomatal size and density. La producción de plantas dihaploides de cebolla (Allium cepa L.), permite obtener líneas completamente homocigotas para luego producir híbridos. A través de la ginogénesis in vitro se ha logrado producir plantas haploides, las cuales deben ser sometidas a tratamientos de duplicación de sus cromosomas, siendo necesario ajustar la concentración del agente duplicador y la duración del tratamiento. En este trabajo se evaluó el efecto de la aplicación de 250 y 500 mg.L-1 de colchicina y 15,2; 30 y 60 mg.L-1 de amiprofos-metil durante 24 y 48 h, sobre la tasa de duplicación de cromosomas en plántulas haploides de cebolla. El mejor tratamiento de duplicación fue 250 mg.L- 1 de colchicina, durante 48 h, logrando un 100% de plantas dihaploides. Por otra parte, se obtuvo una correlación positiva entre el nivel de ploidía y el tamaño de estomas y una correlación negativa entre el nivel de ploidía y la densidad estomática, registrándose diferencias significativas entre los valores promedio de largo, ancho y densidad de estomas para plantas haploides y dihaploides. Se propone un método económico y rápido para verificar el nivel de ploidía de las plántulas de cebolla mediante la medición del tamaño y densidad estomática. |
| titulo_str_mv |
Efecto de la colchicina y del amiprofos-metil en la producción in vitro de plantas dihaploides de cebolla y determinación de la correlación entre el nivel de ploidía y tamaño de los estomas Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| object_type_str_mv |
Textual: Revistas |
| id |
6107 |
| plantilla_str |
Artículo de Revista |
| record_format |
article |
| container_title |
Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
| journal_id_str |
r-78 |
| container_issue |
Revista de la Facultad de Ciencias Agrarias |
| container_volume |
Vol. 45, no. 2 |
| journal_issue_str |
Vol. 45, no. 2 |
| tipo_str |
textuales |
| type_str_mv |
Articulos |
| title_full |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| title_fullStr |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| title_full_unstemmed |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| description |
Doubled haploid onion (Allium cepa L.)
plants allow the production of completely
homozygous lines for a later production of hybrids.
The haploid plants are normally produced using in
vitro gynogenesis. The obtained haploid plantlets
must be treated with different agents for doubling
chromosomes. It is necessary to adjust the
concentration and the length of treatment of the
doubling agent. In this case, the effect of 250 and
500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1
amiprophos-methyl during 24 and 48 h was
assessed over the rate of onion haploid plantlets
chromosome doubling. The best duplication
treatment was 250 mg.L-1 colchicine for 48 h,
which yielded 100% of doubled haploid plants. On
the other hand, a positive correlation resulted from
the ploidy level and stomatal size, and a negative
correlation between the level of ploidy and
stomatal density. Significant differences between
the stomatal length, width and density in haploid
and doubled haploid plantlets were observed.
An economical and quick method to test ploidy
level in onion plantlets is proposed through the
measurement of stomatal size and density. |
| dependencia_str_mv |
Facultad de Ciencias Agrarias |
| title |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| spellingShingle |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size Agentes antimitóticos Allium cepa Cebolla Colchicina Colchicine Duplicación de genes Ginogénesis Gynogenesis Híbridos Hybrids Onions Plantas dihaploides de cebolla Ploidía Ploidy Bohanec, Borut Foschi, María Laura Galmarini, Claudio Martínez, Liliana Ponce, María T. |
| topic |
Agentes antimitóticos Allium cepa Cebolla Colchicina Colchicine Duplicación de genes Ginogénesis Gynogenesis Híbridos Hybrids Onions Plantas dihaploides de cebolla Ploidía Ploidy |
| topic_facet |
Agentes antimitóticos Allium cepa Cebolla Colchicina Colchicine Duplicación de genes Ginogénesis Gynogenesis Híbridos Hybrids Onions Plantas dihaploides de cebolla Ploidía Ploidy |
| author |
Bohanec, Borut Foschi, María Laura Galmarini, Claudio Martínez, Liliana Ponce, María T. |
| author_facet |
Bohanec, Borut Foschi, María Laura Galmarini, Claudio Martínez, Liliana Ponce, María T. |
| title_sort |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| title_short |
Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
| url |
https://bdigital.uncu.edu.ar/fichas.php?idobjeto=6107 |
| estado_str |
3 |
| building |
Biblioteca Digital |
| filtrotop_str |
Biblioteca Digital |
| collection |
Artículo de Revista |
| institution |
Sistema Integrado de Documentación |
| indexed_str |
2023-04-25 00:37 |
| _version_ |
1764120283013185536 |
