Una variante del método de Sordelli para la conservación de cepas microbianas por desecación al vacío
A method for the preservation of microbial cultures in vacuum is described, modifying SORDELLI`s procedure. The modification consists in the use of a single tube of 7 mm. inner diameter and 1 mm. of wall thickness. The culture is introduced and stuck to the bottom of the sterilized tubes, the cotton...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , |
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| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=6956 |
| Sumario: | A method for the preservation of microbial cultures in vacuum is described, modifying SORDELLI`s procedure. The modification consists in the use of a single tube of 7 mm. inner diameter and 1 mm. of wall thickness. The culture is introduced and stuck to the bottom of the sterilized tubes, the cotton plug is pushed and a few pieces of KOH are introduced and compressed between two cotton plugs. The label with the required references is put and the tube is sealed through a previously narrowed place, after a good vacuum is obtained.
The procedure does not modify fundamentally the original method, but has the advantages of the use of a single tube and the guarantee of vacuum permanence. |
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