Un procedimiento para la determinación de la capacidad de intercambio catiónico de raíces
A Procedure for the Determination of the Cation Exchange Capacity of Plant Roots A rapid and simple method for the determination of the exchange capacity of excised plants roots is proposed. The sample, free of foreign substances through a thorough washing, is treated with oxalic acid 0.5N for two...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , |
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| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=7987 |
| Sumario: | A Procedure for the Determination of the Cation Exchange Capacity of Plant Roots
A rapid and simple method for the determination of the exchange capacity of excised plants roots is proposed. The sample, free of foreign substances through a thorough washing, is treated with oxalic acid 0.5N for two successive times during a whole hour. The hydrogenised roots are washed till the elimination of oxalic and then dried between sheets of filter papers. Five grams approximately, of these roots are put into contact in a 200 ml. beaker with 100 ml. of a Ca (OH)2 solution about 0.05N; at the same time another portion of 100 ml. of the Ca (OH)2 solution is poured into another beaker but without roots.
Half an hour later, aliquots of both solutions are removed and their calcium concentration are determined by titration with ethylenediaminetetraacetate (Versenate) in the presence of ammonium purpurate indicator.
The difference between the calcium concentration of the blank solution and the one of the “calcium hydroxide-roots” system is refered to adsorbed calcium and expressed as Cation Exchange Capacity in milliequivalents per 100 grams of oven dried roots.
The procedure proposed does not affect the roots vitality and it proves to be simple, reproductible and adapted to serial determination. |
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