Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr
Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect geneti...
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| Formato: | Online |
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Facultad de Ciencias Agrarias-UNCuyo
2018
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2809 |
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I11-R107article-28092022-11-09T18:13:18Z Analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr Carloni, Edgardo López Colomba , Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina Apomixis embriogénesis somática variación somaclonal cultivo de anteras ISSR diversidad genética Apomixis somatic embryogenesis somaclonal variation anther culture ISSR genetic diversity Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3"² end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants. Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3"² end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants. Facultad de Ciencias Agrarias-UNCuyo 2018-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2809 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 50 No. 2 (2018): July-December; 1-13 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 50 Núm. 2 (2018): Julio-Diciembre; 1-13 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2809/2009 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
| language |
eng |
| format |
Online |
| author |
Carloni, Edgardo López Colomba , Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina |
| spellingShingle |
Carloni, Edgardo López Colomba , Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr Apomixis embriogénesis somática variación somaclonal cultivo de anteras ISSR diversidad genética Apomixis somatic embryogenesis somaclonal variation anther culture ISSR genetic diversity |
| author_facet |
Carloni, Edgardo López Colomba , Eliana Ribotta, Andrea Quiroga, Mariana Tommasino, Exequiel Griffa, Sabrina Grunberg, Karina |
| author_sort |
Carloni, Edgardo |
| title |
Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr |
| title_short |
Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr |
| title_full |
Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr |
| title_fullStr |
Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr |
| title_full_unstemmed |
Análisis de variabilidad genética en plantas regeneradas in vitro de buffelgrass mediante marcadores moleculares issr |
| title_sort |
analysis of genetic variability in in vitro regenerated buffelgrass plants through issr molecular markers |
| description |
Genetic variability can be generated through in vitro culture via somaclonal variation. This tool can be potentially useful in a breeding program involving apomictic buffelgrass genotypes. The aim of this work was to evaluate inter simple sequence repeats (ISSR) as molecular markers to detect genetic variation in in vitro buffelgrass regenerated plants. Six plants regenerated from in vitro anther culture, via somatic embryogenesis were used, as well as the anther donor genotype (RN 51) as control. Of a total of 26 ISSR primers tested, 22 amplified, detecting 12% polymorphism with a divergence between 5 and 24% from RN 51. Amplification products were observed with the primers containing di-, tri- or tetra-nucleotide sequences, with or without additional nucleotides at the 3"² end. The most informative primers were those containing the repetitive sequences GACAn, AGn or GAn. Moreover, the regenerants transplanted at field conditions differed in morphological characteristics among them and with respect to RN 51. This study confirms that ISSR are useful to identify genetic variability in in vitro regenerated buffelgrass plants. |
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2018 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2809 |
| topic |
Apomixis embriogénesis somática variación somaclonal cultivo de anteras ISSR diversidad genética Apomixis somatic embryogenesis somaclonal variation anther culture ISSR genetic diversity |
| topic_facet |
Apomixis embriogénesis somática variación somaclonal cultivo de anteras ISSR diversidad genética Apomixis somatic embryogenesis somaclonal variation anther culture ISSR genetic diversity |
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1800220885713420288 |
