SSR markers linked to stem canker resistance in soybean Glycine max
In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known...
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| Autores principales: | , , |
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| Formato: | Online |
| Lenguaje: | eng |
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Facultad de Ciencias Agrarias-UNCuyo
2020
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933 |
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I11-R107article-2933 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
| language |
eng |
| format |
Online |
| author |
Gilli, Javier Ramón Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia |
| spellingShingle |
Gilli, Javier Ramón Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia SSR markers linked to stem canker resistance in soybean Glycine max Diaporthe phaseolorum var. meridionalis marcadores moleculares resistencia a hongos Glycine max Diaporthe phaseolorum var. meridionalis molecular markers fungal resistance Glycine max |
| author_facet |
Gilli, Javier Ramón Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia |
| author_sort |
Gilli, Javier Ramón |
| title |
SSR markers linked to stem canker resistance in soybean Glycine max |
| title_short |
SSR markers linked to stem canker resistance in soybean Glycine max |
| title_full |
SSR markers linked to stem canker resistance in soybean Glycine max |
| title_fullStr |
SSR markers linked to stem canker resistance in soybean Glycine max |
| title_full_unstemmed |
SSR markers linked to stem canker resistance in soybean Glycine max |
| title_sort |
ssr markers linked to stem canker resistance in soybean glycine max |
| description |
In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known sources of resistance to Dpm. The soybean MJ19RR experimental line showed the best behaviour against this isolate with a DP = 2.4 % and was used to develop a segregating population with the susceptible cultivar FT-2001. In the F2 generation, the chi-square test determined a 3:1 ratio of resistant plants against susceptible plants, as expected for a dominant gene. In order to advance in our study we propose, as objective, to locate in the soybean genetic map the resistance to Diaporthe phaseolorum var. meridionalis. The Bulked Segregant Analyses and the genetic linkage study identified a region of chromosome 6 of the genetic map of soybean, located at 13.3 cM from the Satt433 locus associated with resistance to SCS. The soybean experimental line MJ19RR was selected as the best source of resistance, and it is available in the active bank of soybean germplasm of INTA, for the genetic control of this disease. The results obtained in this work represent a first approximation for the understanding of the genetic basis of resistance to SCS.
Highlights
The PCR sensitivity is a good strategy for detecting markers linkage using bulk segregant analysis.
The MJ19RR soybean genotype is a new promising source of resistance to soybean stem canker.
A single gene determined the genetic resistance to soybean stem canker.
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| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2020 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933 |
| topic |
Diaporthe phaseolorum var. meridionalis marcadores moleculares resistencia a hongos Glycine max Diaporthe phaseolorum var. meridionalis molecular markers fungal resistance Glycine max |
| topic_facet |
Diaporthe phaseolorum var. meridionalis marcadores moleculares resistencia a hongos Glycine max Diaporthe phaseolorum var. meridionalis molecular markers fungal resistance Glycine max |
| work_keys_str_mv |
AT gillijavierramon ssrmarkerslinkedtostemcankerresistanceinsoybeanglycinemax AT velliccegabrielricardo ssrmarkerslinkedtostemcankerresistanceinsoybeanglycinemax AT bernardiclarisanoelia ssrmarkerslinkedtostemcankerresistanceinsoybeanglycinemax |
| _version_ |
1800220887503339520 |
| spelling |
I11-R107article-29332020-07-03T12:12:24Z SSR markers linked to stem canker resistance in soybean Glycine max SSR markers linked to stem canker resistance in soybean Glycine max Gilli, Javier Ramón Vellicce, Gabriel Ricardo Bernardi, Clarisa Noelia Diaporthe phaseolorum var. meridionalis marcadores moleculares resistencia a hongos Glycine max Diaporthe phaseolorum var. meridionalis molecular markers fungal resistance Glycine max In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known sources of resistance to Dpm. The soybean MJ19RR experimental line showed the best behaviour against this isolate with a DP = 2.4 % and was used to develop a segregating population with the susceptible cultivar FT-2001. In the F2 generation, the chi-square test determined a 3:1 ratio of resistant plants against susceptible plants, as expected for a dominant gene. In order to advance in our study we propose, as objective, to locate in the soybean genetic map the resistance to Diaporthe phaseolorum var. meridionalis. The Bulked Segregant Analyses and the genetic linkage study identified a region of chromosome 6 of the genetic map of soybean, located at 13.3 cM from the Satt433 locus associated with resistance to SCS. The soybean experimental line MJ19RR was selected as the best source of resistance, and it is available in the active bank of soybean germplasm of INTA, for the genetic control of this disease. The results obtained in this work represent a first approximation for the understanding of the genetic basis of resistance to SCS. Highlights The PCR sensitivity is a good strategy for detecting markers linkage using bulk segregant analysis. The MJ19RR soybean genotype is a new promising source of resistance to soybean stem canker. A single gene determined the genetic resistance to soybean stem canker. In this work, we studied 40 samples of Diaporthe phaseolorum var. meridionalis (Dpm), causal agent of stem canker in soybeans (SCS). In the susceptible genotype Golondrina65 the isolate RSF12 showed the highest percentage of the dead plant index (DP = 85.7 %) and was used to characterize all known sources of resistance to Dpm. The soybean MJ19RR experimental line showed the best behaviour against this isolate with a DP = 2.4 % and was used to develop a segregating population with the susceptible cultivar FT-2001. In the F2 generation, the chi-square test determined a 3:1 ratio of resistant plants against susceptible plants, as expected for a dominant gene. In order to advance in our study we propose, as objective, to locate in the soybean genetic map the resistance to Diaporthe phaseolorum var. meridionalis. The Bulked Segregant Analyses and the genetic linkage study identified a region of chromosome 6 of the genetic map of soybean, located at 13.3 cM from the Satt433 locus associated with resistance to SCS. The soybean experimental line MJ19RR was selected as the best source of resistance, and it is available in the active bank of soybean germplasm of INTA, for the genetic control of this disease. The results obtained in this work represent a first approximation for the understanding of the genetic basis of resistance to SCS. Highlights The PCR sensitivity is a good strategy for detecting markers linkage using bulk segregant analysis. The MJ19RR soybean genotype is a new promising source of resistance to soybean stem canker. A single gene determined the genetic resistance to soybean stem canker. Facultad de Ciencias Agrarias-UNCuyo 2020-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 No. 1 (2020): January-June; 26-39 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 Núm. 1 (2020): Enero-Junio; 26-39 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933/2096 https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2933/2607 |
