PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri
This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applie...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Online |
| Lenguaje: | eng |
| Publicado: |
Facultad de Ciencias Agrarias-UNCuyo
2023
|
| Materias: | |
| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5309 |
| id |
I11-R107article-5309 |
|---|---|
| record_format |
ojs |
| spelling |
I11-R107article-53092023-09-14T18:51:30Z PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri Maia dos Santos, Ana Paula Santos, Edson Mauro Silva de Oliveira, Juliana Garcia Leal de Araújo, Gherman de Moura Zanine, Anderson Martins Araújo Pinho, Ricardo Costa do Nascimento, Thiago Vinícius Fernandes Perazzo, Alexandre Ferreira, Daniele de Jesus da Silva Macedo, Alberto Jefferson Santos, Francisco Naysson de Sousa fermentación inoculante Lactobacillus plantarum reacción en cadena de la polimerasa fermentation inoculant lactic acid bacteria polymerase chain reaction This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage. Highlights: Lactic acid bacteria showed greater development at 7 days of fermentation. Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population. Lactobacillus buchneri improved its microbiological profile with decreased ethanol production. This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage. Highlights: Lactic acid bacteria showed greater development at 7 days of fermentation. Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population. Lactobacillus buchneri improved its microbiological profile with decreased ethanol production. Facultad de Ciencias Agrarias-UNCuyo 2023-07-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5309 10.48162/rev.39.101 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 55 No. 1 (2023): January-June; 115-125 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 55 Núm. 1 (2023): Enero-Junio; 115-125 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5309/5485 Derechos de autor 2018 Revista de la Facultad de Ciencias Agrarias UNCuyo https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
| institution |
Universidad Nacional de Cuyo |
| building |
Revistas en línea |
| filtrotop_str |
Revistas en línea |
| collection |
Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
| institution_str |
I-11 |
| repository_str |
R-107 |
| language |
eng |
| format |
Online |
| author |
Maia dos Santos, Ana Paula Santos, Edson Mauro Silva de Oliveira, Juliana Garcia Leal de Araújo, Gherman de Moura Zanine, Anderson Martins Araújo Pinho, Ricardo Costa do Nascimento, Thiago Vinícius Fernandes Perazzo, Alexandre Ferreira, Daniele de Jesus da Silva Macedo, Alberto Jefferson Santos, Francisco Naysson de Sousa |
| spellingShingle |
Maia dos Santos, Ana Paula Santos, Edson Mauro Silva de Oliveira, Juliana Garcia Leal de Araújo, Gherman de Moura Zanine, Anderson Martins Araújo Pinho, Ricardo Costa do Nascimento, Thiago Vinícius Fernandes Perazzo, Alexandre Ferreira, Daniele de Jesus da Silva Macedo, Alberto Jefferson Santos, Francisco Naysson de Sousa PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri fermentación inoculante Lactobacillus plantarum reacción en cadena de la polimerasa fermentation inoculant lactic acid bacteria polymerase chain reaction |
| author_facet |
Maia dos Santos, Ana Paula Santos, Edson Mauro Silva de Oliveira, Juliana Garcia Leal de Araújo, Gherman de Moura Zanine, Anderson Martins Araújo Pinho, Ricardo Costa do Nascimento, Thiago Vinícius Fernandes Perazzo, Alexandre Ferreira, Daniele de Jesus da Silva Macedo, Alberto Jefferson Santos, Francisco Naysson de Sousa |
| author_sort |
Maia dos Santos, Ana Paula |
| title |
PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri |
| title_short |
PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri |
| title_full |
PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri |
| title_fullStr |
PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri |
| title_full_unstemmed |
PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri |
| title_sort |
pcr identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated lactobacillus buchneri |
| description |
This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.
Highlights:
Lactic acid bacteria showed greater development at 7 days of fermentation.
Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population.
Lactobacillus buchneri improved its microbiological profile with decreased ethanol production.
|
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2023 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5309 |
| topic |
fermentación inoculante Lactobacillus plantarum reacción en cadena de la polimerasa fermentation inoculant lactic acid bacteria polymerase chain reaction |
| topic_facet |
fermentación inoculante Lactobacillus plantarum reacción en cadena de la polimerasa fermentation inoculant lactic acid bacteria polymerase chain reaction |
| work_keys_str_mv |
AT maiadossantosanapaula pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT santosedsonmauro pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT silvadeoliveirajuliana pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT garcialealdearaujogherman pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT demourazanineanderson pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT martinsaraujopinhoricardo pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT costadonascimentothiagovinicius pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT fernandesperazzoalexandre pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT ferreiradanieledejesus pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT dasilvamacedoalbertojefferson pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri AT santosfrancisconayssondesousa pcridentificationoflacticacidbacteriapopulationsincornsilageinoculatedwithlyophilisedoractivatedlactobacillusbuchneri |
| _version_ |
1800220933916459008 |
