Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene
This research studied serological relationships and genetic diversity of Argentinean isolates of grapevine leafroll-associated virus-4 (GLRaV-4). Phylogenetic analysis of coat protein (CP) sequences from 19 local isolates revealed clustering with the previously described GLRaV-4 strain 5, strain 6,...
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| Autores principales: | , , , , |
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| Formato: | Online |
| Lenguaje: | eng |
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Facultad de Ciencias Agrarias-UNCuyo
2023
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5936 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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R-107 |
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eng |
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Online |
| author |
Gomez Talquenca, Sebastian Lanza Volpe, Melisa Setien, Nancy Gracia, Olga Grau, Oscar |
| spellingShingle |
Gomez Talquenca, Sebastian Lanza Volpe, Melisa Setien, Nancy Gracia, Olga Grau, Oscar Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene caracterización molecular y serología enfermedad del enrollado de la hoja de la vid ampelovirus presión de selección propiedades antigénicas molecular characterization and serology grapevine leafroll disease ampelovirus selection pressure antigenic properties |
| author_facet |
Gomez Talquenca, Sebastian Lanza Volpe, Melisa Setien, Nancy Gracia, Olga Grau, Oscar |
| author_sort |
Gomez Talquenca, Sebastian |
| title |
Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene |
| title_short |
Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene |
| title_full |
Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene |
| title_fullStr |
Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene |
| title_full_unstemmed |
Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene |
| title_sort |
serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene |
| description |
This research studied serological relationships and genetic diversity of Argentinean isolates of grapevine leafroll-associated virus-4 (GLRaV-4). Phylogenetic analysis of coat protein (CP) sequences from 19 local isolates revealed clustering with the previously described GLRaV-4 strain 5, strain 6, and strain 9 groups. Evolutionary sequence analysis of the obtained and database-available sequences showed evidence of recombination events. Additionally, both CP N- and C-terminal regions appeared to be under purifying selection, but the N-terminal region presented seven sites under positive selection, with a dN/dS ratio 5-fold greater than that of the C-terminal region. Serological reactivity against monoclonal antibodies supports a higher occurrence probability for linear epitopes in the N-terminal region, as inferred by the sequence analysis. The obtained results reflect an unusual evolutionary behavior of the CP that, together with protein serological reactivity, suggests biological significance of the observed variability.
Highlights:
The occurrence of GLRaV-4 strains -5, -6 and -9 was recorded in Argentina.
The coat protein sequence showed a highly variable N-terminal region among the strains.
An evolutionary analysis inferred the occurrence of sites under positive diversifying selection pressure.
The most variable region of the coat protein presented the highest probability of occurrence of linear epitopes.
|
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2023 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5936 |
| topic |
caracterización molecular y serología enfermedad del enrollado de la hoja de la vid ampelovirus presión de selección propiedades antigénicas molecular characterization and serology grapevine leafroll disease ampelovirus selection pressure antigenic properties |
| topic_facet |
caracterización molecular y serología enfermedad del enrollado de la hoja de la vid ampelovirus presión de selección propiedades antigénicas molecular characterization and serology grapevine leafroll disease ampelovirus selection pressure antigenic properties |
| work_keys_str_mv |
AT gomeztalquencasebastian serologicalrelationshipsamongstrainsofgrapevineleafrollassociatedvirus4reflecttheevolutivebehaviorofitscoatproteingene AT lanzavolpemelisa serologicalrelationshipsamongstrainsofgrapevineleafrollassociatedvirus4reflecttheevolutivebehaviorofitscoatproteingene AT setiennancy serologicalrelationshipsamongstrainsofgrapevineleafrollassociatedvirus4reflecttheevolutivebehaviorofitscoatproteingene AT graciaolga serologicalrelationshipsamongstrainsofgrapevineleafrollassociatedvirus4reflecttheevolutivebehaviorofitscoatproteingene AT grauoscar serologicalrelationshipsamongstrainsofgrapevineleafrollassociatedvirus4reflecttheevolutivebehaviorofitscoatproteingene |
| _version_ |
1800220943392440320 |
| spelling |
I11-R107article-59362023-09-14T18:49:14Z Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene Serological relationships among strains of grapevine leafroll-associated virus 4 reflect the evolutive behavior of its coat protein gene Gomez Talquenca, Sebastian Lanza Volpe, Melisa Setien, Nancy Gracia, Olga Grau, Oscar caracterización molecular y serología enfermedad del enrollado de la hoja de la vid ampelovirus presión de selección propiedades antigénicas molecular characterization and serology grapevine leafroll disease ampelovirus selection pressure antigenic properties This research studied serological relationships and genetic diversity of Argentinean isolates of grapevine leafroll-associated virus-4 (GLRaV-4). Phylogenetic analysis of coat protein (CP) sequences from 19 local isolates revealed clustering with the previously described GLRaV-4 strain 5, strain 6, and strain 9 groups. Evolutionary sequence analysis of the obtained and database-available sequences showed evidence of recombination events. Additionally, both CP N- and C-terminal regions appeared to be under purifying selection, but the N-terminal region presented seven sites under positive selection, with a dN/dS ratio 5-fold greater than that of the C-terminal region. Serological reactivity against monoclonal antibodies supports a higher occurrence probability for linear epitopes in the N-terminal region, as inferred by the sequence analysis. The obtained results reflect an unusual evolutionary behavior of the CP that, together with protein serological reactivity, suggests biological significance of the observed variability. Highlights: The occurrence of GLRaV-4 strains -5, -6 and -9 was recorded in Argentina. The coat protein sequence showed a highly variable N-terminal region among the strains. An evolutionary analysis inferred the occurrence of sites under positive diversifying selection pressure. The most variable region of the coat protein presented the highest probability of occurrence of linear epitopes. This research studied serological relationships and genetic diversity of Argentinean isolates of grapevine leafroll-associated virus-4 (GLRaV-4). Phylogenetic analysis of coat protein (CP) sequences from 19 local isolates revealed clustering with the previously described GLRaV-4 strain 5, strain 6, and strain 9 groups. Evolutionary sequence analysis of the obtained and database-available sequences showed evidence of recombination events. Additionally, both CP N- and C-terminal regions appeared to be under purifying selection, but the N-terminal region presented seven sites under positive selection, with a dN/dS ratio 5-fold greater than that of the C-terminal region. Serological reactivity against monoclonal antibodies supports a higher occurrence probability for linear epitopes in the N-terminal region, as inferred by the sequence analysis. The obtained results reflect an unusual evolutionary behavior of the CP that, together with protein serological reactivity, suggests biological significance of the observed variability. Highlights: The occurrence of GLRaV-4 strains -5, -6 and -9 was recorded in Argentina. The coat protein sequence showed a highly variable N-terminal region among the strains. An evolutionary analysis inferred the occurrence of sites under positive diversifying selection pressure. The most variable region of the coat protein presented the highest probability of occurrence of linear epitopes. Facultad de Ciencias Agrarias-UNCuyo 2023-07-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5936 10.48162/rev.39.100 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 55 No. 1 (2023): January-June; 104-114 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 55 Núm. 1 (2023): Enero-Junio; 104-114 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5936/5534 Derechos de autor 2018 Revista de la Facultad de Ciencias Agrarias UNCuyo https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
