Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético
Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purpos...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Online |
| Lenguaje: | eng |
| Publicado: |
Facultad de Ciencias Agrarias-UNCuyo
2022
|
| Materias: | |
| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6256 |
| id |
I11-R107article-6256 |
|---|---|
| record_format |
ojs |
| spelling |
I11-R107article-62562022-11-10T17:50:32Z Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: an approach to the appropriate dosage for breeding purposes Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo mutación radio susceptibilidad brotación DL50 mejoramiento genético mutation radio susceptibility sprouting LD50 breeding Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes. Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes. Facultad de Ciencias Agrarias-UNCuyo 2022-11-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6256 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 44 No. 1 (2012): January-June; 191-197 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 44 Núm. 1 (2012): Enero-Junio; 191-197 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6256/5084 https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
| institution |
Universidad Nacional de Cuyo |
| building |
Revistas en línea |
| filtrotop_str |
Revistas en línea |
| collection |
Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
| institution_str |
I-11 |
| repository_str |
R-107 |
| language |
eng |
| format |
Online |
| author |
Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo |
| spellingShingle |
Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético mutación radio susceptibilidad brotación DL50 mejoramiento genético mutation radio susceptibility sprouting LD50 breeding |
| author_facet |
Aros, Danilo Valdés, Santiago Olate, Eduardo Infante, Rodrigo |
| author_sort |
Aros, Danilo |
| title |
Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético |
| title_short |
Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético |
| title_full |
Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético |
| title_fullStr |
Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético |
| title_full_unstemmed |
Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: una aproximación a la dosis apropiada para utilizar en mejoramiento genético |
| title_sort |
gamma irradiation on alstroemeria aurea g. in vitro rhizomes: an approach to the appropriate dosage for breeding purposes |
| description |
Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding purposes and it depends mainly on plant susceptibility. Thus in this study in vitro cultured rhizomes of Alstroemeria aurea were irradiated with a gamma source using different dosages to evaluate the direct effect produced. Damage and number of rhizome sprouting were observed and recorded during 61 days after irradiation. At the end of this period, rhizomes were weighted and mortality was evaluated. Both mortality and weight increased depending on dosage. All irradiated rhizomes showed early sprouting in comparison with control (0 Gy) and no significant difference in final number of shoots after 61 days among irradiated treatments was observed. Bleaching and necrosis was observed in all irradiated rhizomes and was more evident at higher doses. LD50 was established at about 40 Gy and the optimum dosage to induce mutation was suggested between 2.5 and 5 Gy, when the growth was reduced in 50%, and probably this dosage could be used for breeding purposes. |
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2022 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6256 |
| topic |
mutación radio susceptibilidad brotación DL50 mejoramiento genético mutation radio susceptibility sprouting LD50 breeding |
| topic_facet |
mutación radio susceptibilidad brotación DL50 mejoramiento genético mutation radio susceptibility sprouting LD50 breeding |
| work_keys_str_mv |
AT arosdanilo gammairradiationonalstroemeriaaureaginvitrorhizomesanapproachtotheappropriatedosageforbreedingpurposes AT valdessantiago gammairradiationonalstroemeriaaureaginvitrorhizomesanapproachtotheappropriatedosageforbreedingpurposes AT olateeduardo gammairradiationonalstroemeriaaureaginvitrorhizomesanapproachtotheappropriatedosageforbreedingpurposes AT infanterodrigo gammairradiationonalstroemeriaaureaginvitrorhizomesanapproachtotheappropriatedosageforbreedingpurposes AT arosdanilo irradiacionderizomasinvitrodealstroemeriaaureagconrayosgammaunaaproximacionaladosisapropiadaparautilizarenmejoramientogenetico AT valdessantiago irradiacionderizomasinvitrodealstroemeriaaureagconrayosgammaunaaproximacionaladosisapropiadaparautilizarenmejoramientogenetico AT olateeduardo irradiacionderizomasinvitrodealstroemeriaaureagconrayosgammaunaaproximacionaladosisapropiadaparautilizarenmejoramientogenetico AT infanterodrigo irradiacionderizomasinvitrodealstroemeriaaureagconrayosgammaunaaproximacionaladosisapropiadaparautilizarenmejoramientogenetico |
| _version_ |
1800220951578673152 |
