A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina
Maize (Zea mays L.) is one of the most widely planted crops globally with Argentina leading world production and exportation. Santiago del Estero province, east of Tucumán and north of Córdoba encompasses eight agro-climatic zones in the Chaco Semi-arid region, agro-ecologically characterized by a...
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| Formato: | Online |
| Lenguaje: | eng |
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Facultad de Ciencias Agrarias-UNCuyo
2024
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| Materias: | |
| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6970 |
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I11-R107article-6970 |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
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eng |
| format |
Online |
| author |
Barontini, Javier Alaniz Zanon, María Silvina Torrico, Ada Karina Druetta, Marcelo Luna, Ignacio Martín Ruiz Posse, Agustina Chulze, Sofía Noemí Giménez Pecci, María de la Paz |
| spellingShingle |
Barontini, Javier Alaniz Zanon, María Silvina Torrico, Ada Karina Druetta, Marcelo Luna, Ignacio Martín Ruiz Posse, Agustina Chulze, Sofía Noemí Giménez Pecci, María de la Paz A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina aislado no aflatoxigénico ácido ciclopiazónico producción de esclerocios relaciones filogenéticas non-aflatoxigenic isolate cyclopiazonic acid sclerotia production phylogenetic relationships |
| author_facet |
Barontini, Javier Alaniz Zanon, María Silvina Torrico, Ada Karina Druetta, Marcelo Luna, Ignacio Martín Ruiz Posse, Agustina Chulze, Sofía Noemí Giménez Pecci, María de la Paz |
| author_sort |
Barontini, Javier |
| title |
A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina |
| title_short |
A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina |
| title_full |
A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina |
| title_fullStr |
A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina |
| title_full_unstemmed |
A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina |
| title_sort |
polyphasic study of non-aflatoxigenic aspergillus flavus link, isolates from maize in the chaco semi-arid region of argentina |
| description |
Maize (Zea mays L.) is one of the most widely planted crops globally with Argentina leading world production and exportation. Santiago del Estero province, east of Tucumán and north of Córdoba encompasses eight agro-climatic zones in the Chaco Semi-arid region, agro-ecologically characterized by a wide temperature range and frequent drought periods that expose the crop to pathogens, particularly Aspergillus flavus. This pathogen is responsible for ear rot and grain contamination with mycotoxins such as aflatoxin B1 and cyclopiazonic acid. This study obtained fungal isolates from ears of maize and characterized them according to toxigenic capability and morphotype of sclerotia (S < 400 μm, associated with high levels of aflatoxins and L > 400 μm, related to variable levels of aflatoxins). In addition, those not producing aflatoxins were studied to determine phylogenetic relationships based on sequences of a segment of the CaM gene. Fifty-eight isolates were obtained in eight localities representing each agro-climatic zone, 30 of which were non-aflatoxigenic, 28 aflatoxigenic, and all producers of ciclopiazonic acid. Six isolates did not produce sclerotia, 51 were L and only one was S, the latter being a non-producer of aflatoxins. The number of sclerotia was positively correlated with the production of aflatoxin B1, while size was negatively correlated. The CaM gene sequences corroborated that the isolates belonged to the A. flavus clade and the high nucleotide similarity among them (99.4% to 100%) revealed almost zero genetic diversity in this geographic region. No significant differences were observed in the proportion of isolates between growing seasons or among agroclimatic districts. This research revealed characteristics of fungus populations in this agricultural region of north Argentina.
Highlights:
Aspergillus flavus isolates from ears of maize were characterized according to toxigenic capability, morphotype of sclerotia and phylogenetic relationships.
Fifty-eight isolates were obtained, 30 non-aflatoxigenic, 28 aflatoxigenic, and all producers of ciclopiazonic acid.
Six isolates did not produce sclerotia, 51 were L and only one was S, the latter being a non-producer of aflatoxins.
All isolates belonged to the flavus clade. High nucleotide similarity among the isolates (99.4% to 100%) revealed almost zero genetic diversity in the region.
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| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2024 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6970 |
| topic |
aislado no aflatoxigénico ácido ciclopiazónico producción de esclerocios relaciones filogenéticas non-aflatoxigenic isolate cyclopiazonic acid sclerotia production phylogenetic relationships |
| topic_facet |
aislado no aflatoxigénico ácido ciclopiazónico producción de esclerocios relaciones filogenéticas non-aflatoxigenic isolate cyclopiazonic acid sclerotia production phylogenetic relationships |
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1800221038657667072 |
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I11-R107article-69702024-05-24T13:06:34Z A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina A polyphasic study of non-aflatoxigenic Aspergillus flavus Link, isolates from maize in the Chaco semi-arid region of Argentina Barontini, Javier Alaniz Zanon, María Silvina Torrico, Ada Karina Druetta, Marcelo Luna, Ignacio Martín Ruiz Posse, Agustina Chulze, Sofía Noemí Giménez Pecci, María de la Paz aislado no aflatoxigénico ácido ciclopiazónico producción de esclerocios relaciones filogenéticas non-aflatoxigenic isolate cyclopiazonic acid sclerotia production phylogenetic relationships Maize (Zea mays L.) is one of the most widely planted crops globally with Argentina leading world production and exportation. Santiago del Estero province, east of Tucumán and north of Córdoba encompasses eight agro-climatic zones in the Chaco Semi-arid region, agro-ecologically characterized by a wide temperature range and frequent drought periods that expose the crop to pathogens, particularly Aspergillus flavus. This pathogen is responsible for ear rot and grain contamination with mycotoxins such as aflatoxin B1 and cyclopiazonic acid. This study obtained fungal isolates from ears of maize and characterized them according to toxigenic capability and morphotype of sclerotia (S < 400 μm, associated with high levels of aflatoxins and L > 400 μm, related to variable levels of aflatoxins). In addition, those not producing aflatoxins were studied to determine phylogenetic relationships based on sequences of a segment of the CaM gene. Fifty-eight isolates were obtained in eight localities representing each agro-climatic zone, 30 of which were non-aflatoxigenic, 28 aflatoxigenic, and all producers of ciclopiazonic acid. Six isolates did not produce sclerotia, 51 were L and only one was S, the latter being a non-producer of aflatoxins. The number of sclerotia was positively correlated with the production of aflatoxin B1, while size was negatively correlated. The CaM gene sequences corroborated that the isolates belonged to the A. flavus clade and the high nucleotide similarity among them (99.4% to 100%) revealed almost zero genetic diversity in this geographic region. No significant differences were observed in the proportion of isolates between growing seasons or among agroclimatic districts. This research revealed characteristics of fungus populations in this agricultural region of north Argentina. Highlights: Aspergillus flavus isolates from ears of maize were characterized according to toxigenic capability, morphotype of sclerotia and phylogenetic relationships. Fifty-eight isolates were obtained, 30 non-aflatoxigenic, 28 aflatoxigenic, and all producers of ciclopiazonic acid. Six isolates did not produce sclerotia, 51 were L and only one was S, the latter being a non-producer of aflatoxins. All isolates belonged to the flavus clade. High nucleotide similarity among the isolates (99.4% to 100%) revealed almost zero genetic diversity in the region. Maize (Zea mays L.) is one of the most widely planted crops globally with Argentina leading world production and exportation. Santiago del Estero province, east of Tucumán and north of Córdoba encompasses eight agro-climatic zones in the Chaco Semi-arid region, agro-ecologically characterized by a wide temperature range and frequent drought periods that expose the crop to pathogens, particularly Aspergillus flavus. This pathogen is responsible for ear rot and grain contamination with mycotoxins such as aflatoxin B1 and cyclopiazonic acid. This study obtained fungal isolates from ears of maize and characterized them according to toxigenic capability and morphotype of sclerotia (S < 400 μm, associated with high levels of aflatoxins and L > 400 μm, related to variable levels of aflatoxins). In addition, those not producing aflatoxins were studied to determine phylogenetic relationships based on sequences of a segment of the CaM gene. Fifty-eight isolates were obtained in eight localities representing each agro-climatic zone, 30 of which were non-aflatoxigenic, 28 aflatoxigenic, and all producers of ciclopiazonic acid. Six isolates did not produce sclerotia, 51 were L and only one was S, the latter being a non-producer of aflatoxins. The number of sclerotia was positively correlated with the production of aflatoxin B1, while size was negatively correlated. The CaM gene sequences corroborated that the isolates belonged to the A. flavus clade and the high nucleotide similarity among them (99.4% to 100%) revealed almost zero genetic diversity in this geographic region. No significant differences were observed in the proportion of isolates between growing seasons or among agroclimatic districts. This research revealed characteristics of fungus populations in this agricultural region of north Argentina. Highlights: Aspergillus flavus isolates from ears of maize were characterized according to toxigenic capability, morphotype of sclerotia and phylogenetic relationships. Fifty-eight isolates were obtained, 30 non-aflatoxigenic, 28 aflatoxigenic, and all producers of ciclopiazonic acid. Six isolates did not produce sclerotia, 51 were L and only one was S, the latter being a non-producer of aflatoxins. All isolates belonged to the flavus clade. High nucleotide similarity among the isolates (99.4% to 100%) revealed almost zero genetic diversity in the region. Facultad de Ciencias Agrarias-UNCuyo 2024-05-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6970 Revista de la Facultad de Ciencias Agrarias UNCuyo; In press; XXX-XXX Revista de la Facultad de Ciencias Agrarias UNCuyo; En prensa; XXX-XXX 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/6970/6363 Derechos de autor 2018 Revista de la Facultad de Ciencias Agrarias UNCuyo https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
