Regeneración de plantas de té (Camellia sinensis) por cultivo in vitro de meristemas, yemas axilares y segmentos uninodales
Plants of two clones (CH 14 INTA and CH 318 INTA) of tea (Camellia sinensis (L.) O. Kuntze) were regenerated by in vitro culture of three types of explants disinfected by immersion in 70% ethanol (1 min) and 1.5% sodium hypochlorite (20 min). The best medium for shoot regeneration from uninoda...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=5012 |
| Sumario: | Plants of two clones (CH 14 INTA
and CH 318 INTA) of tea (Camellia sinensis (L.)
O. Kuntze) were regenerated by in vitro culture of
three types of explants disinfected by immersion
in 70% ethanol (1 min) and 1.5% sodium
hypochlorite (20 min). The best medium for shoot
regeneration from uninodal segments, for both
clones, as well as for axillary buds of CH 14 INTA
clone was ½ MS + 1 mg/L BAP. While the best
medium for axillary buds of CH 318 clone
was ½ MS + 1 mg/L BAP + 1 mg/L AG3. For
meristems culture, the best medium, for both
clones was ½ MS + 1 mg/L KIN + 1 mg/L AG3.
Rooting of regenerated shoots were achieved by
culture them on ¼ MS + 6 mg/L IBA.
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